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整合REV LTR的自然重组马立克氏病毒GX0101的全基因组序列分析
周忠文,苏帅,崔宁,孙鹏,孙淑红,崔治中
0
(山东农业大学动物科技学院)
摘要:
GX0101是一株整合禽网状内皮组织增生症病毒 (reticuloendotheliosis, REV) 长末端重复序列 (LTR) 的重组马立克氏病病毒 (Marek′s disease, MD)。本研究基于MDV GX0101的细菌人工染色体感染性克隆利用高通量测序技术完成了对其全基因组的序列分析。GX0101全基因组长178101bp, 其基因组的TRL、UL、IRL、IRS、US和TRS区分别长12758bp、113572bp、12741bp、12700bp、11695bp、13134bp。GX0101全基因组仅含有一个REV LTR重组片段,位于其基因组US区的sorf1中,sorf2基因前267bp碱基处。与rMd5不同,GX0101含有一个sorf2基因。通过与已发表的近10株MDV的比较, 发现GX0101与英国分离株C12/130的两个不同毒力的感染性克隆pC12/130-10和pC12/130-15同源性最高。GX0101的全基因组序列的比较分析,有助于进一步阐明MDV致病性、传播性相关的基因,也有助于揭示不同地域间MDV的遗传变异和演化关系。
关键词:  REV LTR  重组MDV  感染性克隆  高通量测序  全基因组
DOI:
投稿时间:2017-06-29修订日期:2017-08-03
基金项目:国家自然科学基金青年科学基金项目(31402235)
Complete genome sequence analysis of a recombinant marek’s disease virus field strain, GX0101, with a reticuloendotheliosis virus LTR insert
(Animal Science and Technology College,Shandong Agricultural University,Shandong,Tai‘an)
Abstract:
GX0101 is a recombinant Marek’s disease virus (MDV) field strain with one reticuloendotheliosis virus (REV) long terminal repeat (LTR) insert. On the basis of the purified plasmid DNA of GX0101 bacterial artificial chromosome (BAC) infectious clone, the complete genome of GX0101 was sequenced and analyzed using high throughput sequencing technology. The complete genome sequence of GX0101 is 178,101 bp in length, and the terminal repeat long (TRL), unique long (UL), internal repeat long (IRL), internal repeat short (IRS), unique short (US), and terminal repeat short (TRS) regions with the lengths of 12,758, 113,572, 12,741, 12,700, 11,695, and 13,134 bp, respectively. The GX0101 genome contains only one REV LTR insert located in sorf1 gene of US region, at a site 267 bp upstream of the sorf2 gene. Compared to rMd5, GX0101 contains only one sorf2 gene. Among the 10 recently reported MDV strains, GX0101 has the highest identity to two BAC clones PC12130-10 and PC12130-15 of United Kingdom strain C12130 with different virulence. Analysis of the complete sequence of GX0101 will be useful in both studies of gene functions related to pathogenicity or transmission ability and understanding genomic mutations or evolutionary relationships of MDVs in different geographical areas.
Key words:  REV LTR  recombinant MDV  infectious clone  high throughput sequencing  complete genome

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