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鸡痘病毒活疫苗污染禽网状内皮组织增生症病毒的检测
孙淼,李岭,李启红,夏业才,李慧姣,李俊平,杨承槐
0
(中国兽医药品监察所)
摘要:
通过向无外源病毒污染的鸡痘病毒活疫苗中添加不同剂量的禽网状内皮组织增生症病毒(Reticuloendotheliosis virus, REV),然后用间接免疫荧光法(indirect immunoinfluorescence assay, IFA)进行检测,确定了该IFA方法的最低检出量为每500羽份疫苗中污染20 TCID50的REV。使用该方法对国内16家企业生产的60批鸡痘病毒活疫苗进行了检验,结果显示2个企业生产的4批疫苗REV检测为阳性。随机选取5批IFA检测阴性样品和4批IFA检测阳性样品,按鸡检查法进行外源病毒检验,结果两种方法对REV污染的检测结果的符合率为100%。
关键词:  鸡痘病毒活疫苗  REV  IFA
DOI:
投稿时间:2017-06-12修订日期:2017-07-20
基金项目:
Detection of Avian reticuloendotheliosis virus contamination in fowlpox live vaccine
(China Institute of Veterinary Drug Control)
Abstract:
In this study, the fowlpox live vaccine, not contaminated with exogenous viruses, was added with different doses of avian reticuloendotheliosis virus (REV) and REV was detected by indirect immunofluorescence assay (IFA). The results indicated that the lowest detection limit was 20TCID50 for 500 doses of vaccine. 60 batches of fowlpox live vaccine from 16 companies were selected to detect REV contamination using IFA method. The results showed 4 batches of vaccine were REV positive. 5 batches of REV negative vaccine and 4 batches of REV positive vaccine were selected randomly for exogenous virus detection using chicken test method. The coincidence rate of the two methods was 100%.
Key words:  fowlpox live vaccine  REV  IFA

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