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维氏气单胞菌RCR快速检测方法的建立及初步应用
边宇,单晓枫
0
(吉林农业大学动物科学技术学院)
摘要:
以维氏气单胞菌的核酸酶基因为靶基因建立了PCR快速检测方法。实验证明该方法具有良好的特异性与敏感性,对维氏气单胞菌的最低检测浓度为1.58*10-4 ng;人工模拟污染试验显示:该方法的样本检出率达到了86.7%,高于细菌分离培养的检出率73.3%。该方法的建立,为维氏气单胞菌的快速检测提供新的方法。
关键词:  维氏气单胞菌  聚合酶链式反应  核酸酶
DOI:
投稿时间:2012-08-01修订日期:2012-10-30
基金项目:国家支撑计划项目(2010BAD04B01);吉林省科技厅项目(20080218);吉林农业大学青年启动基金(200904)
Establishment and Preliminary Application of Rapid Detection Method for Aeromonas Veronii by PCR
(College of Animal Science and Technology of Jilin Agricultural University)
Abstract:
Rapid Detection Method by PCR for Aeromonas Veronii was established by nuclease gene regarded as the target gene in this study. The results showed that the method had good specificity and sensitivity and the minimum detectable concentration to A.veronii was 1.58 ? 10-4 ng. Trials of artificial simulated pollution showed that the detectable rate of this method reached 86.7% and it was higher than the rate of 73.3% by bacteria cultivation. The establishment of the method provides a new way for rapidly detecting A.veronii..
Key words:  Aeromonas Veronii  PCR  Nuclease

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